EZ DNA Methylation-Direct Kit

Description

A complete and reliable bisulfite conversion kit that eliminates cumbersome DNA precipitation steps and allows DNA bisulfite conversion directly from blood, tissue, and cells without prior DNA purification.

Feature

• Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
• Compatible with small sample inputs as few as 10 cells or 50 pg of DNA.
• DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Overview

The EZ DNA Methylation-Direct Kit is a further refinement of our popular EZ DNA Methylation and EZ DNA Methylation-Gold kits. This bisulfite conversion kit features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.

Specificaiton

• Elution Volume:≥ 10 ul
• Size Range: 50 bp to ~ 23 kb
• Equipment: Thermocycler with heated lid and microcentrifuge.
• Purity: Purified, converted DNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
• Sample Source: Blood, tissue, cells, FFPE, LCM-derived samples as well as purified plasmid, genomic and endonuclease digested DNAs.
• Applicable For: Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
• Processing Time: 4 hours
• Input: Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-10⁵ cells.
• Conversion: >99.5%
• Recovery:> 80%

Technical Information & Protocol