EZ DNA Methylation-Gold Kit

$305.00$1,025.00

$305.00
$1,025.00

Description

Complete bisulfite conversion of GC-rich DNA in less than 3 hours.

Feature

  • Complete bisulfite conversion of GC-rich DNA in less than 3 hours.
  • A coupled heat denaturation/conversion reaction step steamlines the conversion of non-methylated cytosines into uracil.
  • Desulfonation and recovery of bisulfite-treated DNA with a spin column.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Overview

The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation instead of chemical denaturation which is used in the original EZ DNA Methylation™ Kit. Also, the EZ DNA Methylation-Gold™ Kits have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Specification

  • Elution Volume:≥ 10 ul
  • Size Range:50 bp to ~ 23 kb
  • Equipment: Thermocycler with heated lid, heat block or water bath and microcentrifuge.
  • Purity: Purified, converted DNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
  • Sample Source: Purified plasmid, genomic and endonuclease digested DNAs. DNA should be of the highest quality and RNA-free.
  • Applicable For: Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
  • Processing Time: 3 hours
  • Input: 500 pg – 2 µg of DNA.

Technical Information & Protocol

Product SKU Format Elution Volume Input Protocol SDS
EZ DNA Methylation-Gold™ D5005 Spin-Column ≥ 10 ul 500 pg – 2 µg of DNA. Protocol SDS (MSDS)
EZ-96 DNA Methylation-Gold™ Kit D5007 96-Well (Shallow) ≥ 30 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)
EZ-96 DNA Methylation-Gold™ Kit D5008 96-Well (Deep) ≥ 15 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)

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