EZ DNA Methylation Kit

$285.00$979.00

$285.00
$979.00

Description

Streamlined bisulfite treatment of DNA.

Feature

  • Streamlined, proven procedure for bisulfite conversion of DNA.
  • Desulphonation and recovery of bisulfite-treated DNA with a spin column.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Overview

The EZ DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA. This kit is the original bisulfite conversion kit from Zymo Research. The EZ DNA Methylation™ Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. Designed to reduce template degradation, this kit minimizes DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. These kits are recommended with Illumina’s GoldenGate® and Infinium® Assays.

Specification

  • Size Range:50 bp to ~ 23 kb
  • Equipment: Thermocycler with heated lid, heat block or water bath and microcentrifuge.
  • Purity:Purified, converted DNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
  • Sample Source: Purified plasmid, genomic and endonuclease digested DNAs. DNA should be of the highest quality and RNA-free.
  • Applicable For: Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. This kit is validated for use with Illumina’s GoldenGate® and Infinium® Assays.
  • Processing Time: 12-16 hours
  • Input: 500 pg – 2 µg of DNA.
  • Conversion:>99%
  • Recovery: >80%

Technical Information & Protocol

Product SKU Format Elution Volume Input Protocol SDS
EZ DNA Methylation™ Kit D5001 Spin-Column ≥ 10 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)
EZ-96 DNA Methylation™ Kit D5003 96-Well (Shallow) ≥ 30 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)
EZ-96 DNA Methylation™ Kit D5004 96-Well (Deep) ≥ 15 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)
EZ-96 DNA Methylation™ MagPrep D5040 Magnetic Beads ≥ 25 µl 500 pg – 2 µg of DNA. Protocol SDS (MSDS)

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