GenJet™ In Vitro siRNA Transfection Reagent (Ver. II)

Description

In Vitro siRNA Transfection Reagents:

Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, has a well-defined structure: a short (usually 21-nt) double strand of RNA (dsRNA) with 2-nt 3′ overhangs on either end. siRNAs can be introduced into cells by various transfection methods to bring about the specific knockdown of a gene of interest. Essentially any gene of which the sequence is known can thus be targeted based on sequence complementarity with an appropriately tailored siRNA. This has made siRNAs an important and indispensable tool for gene function and drug target validation studies in the post-genomic era.

While liposome or polymer reagents often give very good DNA delivery efficacy, they often failed to efficiently drive siRNA into mammalian cells due to the length of the siRNA anionic segment that is too short to maintain electrostatic cohesion with the cationic lipids or polycationic polymer. We modified the liposome and polymer by addition of specific pre-screened hydrophobic groups which confers the pH dependent conformational changes (PDCC) at physiological pH condition and greatly stabilizes siRNA lipolplex or polyplex.

Based on our unique and proprietary “PDCC” technology, currently we are developing and manufacturing the following four siRNA transfection reagents, which show distinct transfection characteristics with the leading products in the market.

siRNA Transfection Reagent Selection Chart.

Reagents	Features	Transfection Type	Toxicity
PepMute™	– Exceptional good for siRNA transfection – OK for DNA/siRNA co-transfection	siRNA DNA/siRNA	++
GenMute™	– Exceptional good for siRNA transfection – Excellent for DNA/siRNA co-transfection	siRNA DNA/siRNA	++
PowerFect™	– OK for siRNA transfection – Excellent for DNA transfection – Excellent for DNA/siRNA co-transfection	DNA DNA/siRNA	+
PepMute™ Plus	– Exceptional good for siRNA transfection – Excellent for DNA/siRNA co-transfection	siRNA DNA/siRNA	++

Catalog #: SL100570

Unit Size: 1.0 mL

Description:
GenJet™ siRNA Transfection Reagent, a hybrid of liposome and polymer, ensures superior efficiency siRNA delivery with invisible cytotoxicity. Based on innovative and proprietary lipid-conjugation and polymer synthesis technology and formulation, this product exhibits significant difference from other siRNA transfection reagents in the market.

Size & content:
– GenJet™ siRNA Reagent, 1.0 mL, sufficient for ~1330 reactions based on transfecting 5.0 pmol siRNA in 24-well plate.
– GenJet™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL of working solution.

Storage:
Store at 4 °C for GenJet™ Reagent and RT for GenJet™ Transfection Buffer (5x ).  If stored properly, the product is stable for 12 months or longer.

Advantages: 
Based on innovative and proprietary lipid-conjugation and polymer synthesis technology and formulation, this product exhibits significant difference from other siRNA transfection reagents in the market:

• SUPERIOR EFFICIENCY 
– Top choice for difficult-to-transfect cells
– Best for broad mammalian cells
– Invisible cytotoxicity

• LESS EXPENSIVE

• MORE ADVANTAGES
– No sera interference
– Very stable
– Simple and quick
– Suitable for high-throughput application…

Data Sheet & Protocol